Journal: Frontiers in Immunology

Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

doi: 10.3389/fimmu.2025.1528520

Figure Lengend Snippet: CBD treatment promotes anti-cancer activity in HPV-POSITIVE HNSCC cells. Measurement of cell proliferation by AquaBluer Assay after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 24 and 48 hours. Graphical representations of percentage of cell proliferation by BrdU assay on (C) UPCI: SCC154 and (D) UD-SCC-2 after treatment with CBD 10 μM for a time period of 24 h and 48h. Cell proliferation decreases significantly with CBD treatment compared to Vehicle treated group. Measurement of percentage of apoptosis post CBD treatment for 48 hours in HPV-positive HNSCC cells (E) UPCI: SCC154 and (F) UD-SCC-2. Measurement of percentage of cellular migration post CBD treatment for 48 hours in HPV-positive cells (G) UPCI: SCC154 and (H) UD-SCC-2. Statistical analysis was performed by unpaired Student’s t-test. [*p<0.05 **p<0.01].

Article Snippet: The human HPV positive HNSCC cell line UPCI: SCC154 was purchased from American Type Culture Collection (#CRL-3241, ATCC) and UD-SCC-2 (RRID: CVCL_E325) was obtained from the Dr. Silvio Gutkind’s Laboratory at the Moores Cancer Center, University of California San Diego.

Techniques: Activity Assay, BrdU Staining, Migration

Journal: Frontiers in Immunology

Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

doi: 10.3389/fimmu.2025.1528520

Figure Lengend Snippet: CBD treatment promote activation of markers of MAPK pathway in HPV-positive HNSCC cells. Western blot analysis of MAPK pathway markers like p38, ERK1/2, JNK/SAPK and MK2 and it’s signaling after treatment with 10 μM of CBD in HPV-positive HNSCC cells (A) UPCI: SCC154 and (B) UD-SCC-2 for 30 minutes.

Article Snippet: The human HPV positive HNSCC cell line UPCI: SCC154 was purchased from American Type Culture Collection (#CRL-3241, ATCC) and UD-SCC-2 (RRID: CVCL_E325) was obtained from the Dr. Silvio Gutkind’s Laboratory at the Moores Cancer Center, University of California San Diego.

Techniques: Activation Assay, Western Blot

Journal: Frontiers in Immunology

Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

doi: 10.3389/fimmu.2025.1528520

Figure Lengend Snippet: CBD treatment promotes anti-tumor activity in syngeneic mouse models of HNSCC. Tumor volume of mice injected with 1x10 6 mEER cells/mouse in the flank region of (A) immunocompetent syngeneic C57BL/6J mice model (wild-type), (B) athymic nude mice and (C) Rag1 KO. From Day 6, the mice were treated with 10 μM of CBD and vehicle control (1:9 DMSO: PBS) intraperitoneally for everyday and continued till Day 21. Statistical analysis was performed by unpaired Student’s t-test [*p<0.05].

Article Snippet: The human HPV positive HNSCC cell line UPCI: SCC154 was purchased from American Type Culture Collection (#CRL-3241, ATCC) and UD-SCC-2 (RRID: CVCL_E325) was obtained from the Dr. Silvio Gutkind’s Laboratory at the Moores Cancer Center, University of California San Diego.

Techniques: Activity Assay, Injection, Control

Journal: Frontiers in Immunology

Article Title: CBD promotes antitumor activity by modulating tumor immune microenvironment in HPV associated head and neck squamous cell carcinoma

doi: 10.3389/fimmu.2025.1528520

Figure Lengend Snippet: CBD treatment facilitates CD4+T and CD8+ T cells mediated anti-tumor immune response in syngeneic mouse models of HNSCC. (A) Tumor volume of mice injected with 1x10 6 mEER cells/mouse in the flank region of C57BL/6J mice model (wild-type). The mice were treated with vehicle control, 10 µM of CBD, anti-CD4 and its combination intraperitoneally for everyday and continued till Day 21. (B) . Tumor volume of mice injected with 1x10 6 mEER cells/mouse in the flank region of C57BL/6J mice model (wild-type) and treated with vehicle control, 10 µM of CBD, anti-CD8 and its combination. (C) Tumor volume of mice treated with IgG antibody in the presence and absence of 10 µM of CBD. Statistical analysis was performed by unpaired Student’s t-test [*p<0.05 **p<0.01, ***p<0.001].

Article Snippet: The human HPV positive HNSCC cell line UPCI: SCC154 was purchased from American Type Culture Collection (#CRL-3241, ATCC) and UD-SCC-2 (RRID: CVCL_E325) was obtained from the Dr. Silvio Gutkind’s Laboratory at the Moores Cancer Center, University of California San Diego.

Techniques: Injection, Control

Journal: OncoTargets and therapy

Article Title: DNMT1 Enhances the Radiosensitivity of HPV-Positive Head and Neck Squamous Cell Carcinomas via Downregulating SMG1

doi: 10.2147/OTT.S227395

Figure Lengend Snippet: HPV-positive HNSCC cell lines are more sensitive to radiation than HPV-negative HNSCC cell lines. ( A ) The mRNA expressions of E6, E7, p16, p53 in FaDu, UM-SCC-4, UM-SCC-47 and UPCI-SCC-090 cell lines by RT-qPCR. ( B ) The protein expressions of E6, E7, p16, p53 in FaDu, UM-SCC-4, UM-SCC-47 and UPCI-SCC-090 cell lines by Western blot. ( C, D, E ) The different sensitivity to radiation among the FaDu, UM-SCC-4, UM-SCC-47 and UPCI-SCC-090 cell lines. *P < 0.05.

Article Snippet: We used 2 HPV-negative (FaDu, UM-SCC-4) and 2 HPV-positive (UPCI-SCC-090, UM-SCC-47) HNSCC cell lines which were bought from ATCC immortalized by expression of hTERT.

Techniques: Quantitative RT-PCR, Western Blot

Journal: OncoTargets and therapy

Article Title: DNMT1 Enhances the Radiosensitivity of HPV-Positive Head and Neck Squamous Cell Carcinomas via Downregulating SMG1

doi: 10.2147/OTT.S227395

Figure Lengend Snippet: HPV-16 E6 promotes the sensitivity of radiation in HNSCC cells. ( A ) The mRNA expression of E6 and p16 when E6 overexpression in the FaDu and UM-SCC-4 cells was detected by RT-qPCR. ( B ) The protein expressions of E6 and p16 when E6 overexpression in the FaDu and UM-SCC-4 cells was detected by Western blot. ( C ) The sensitivity of the cells to radiation was detected by clonogenic survival assay when E6 overexpression in the FaDu and UM-SCC-4 cells. ( D ) The mRNA expression of E6 and p16 when E6 silenced in UM-SCC-47 and UPCI-SCC-090 cells were detected by RT-qPCR. ( E ) The protein expressions of E6 and p16 when E6 silenced in UM-SCC-47 and UPCI-SCC-090 cells were detected by Western blot. ( F ) The sensitivity of the cells to radiation was detected by clonogenic survival assay when E6 silenced UM-SCC-47 and UPCI-SCC-090 cells. *P < 0.05.

Article Snippet: We used 2 HPV-negative (FaDu, UM-SCC-4) and 2 HPV-positive (UPCI-SCC-090, UM-SCC-47) HNSCC cell lines which were bought from ATCC immortalized by expression of hTERT.

Techniques: Expressing, Over Expression, Quantitative RT-PCR, Western Blot, Clonogenic Cell Survival Assay

Journal: OncoTargets and therapy

Article Title: DNMT1 Enhances the Radiosensitivity of HPV-Positive Head and Neck Squamous Cell Carcinomas via Downregulating SMG1

doi: 10.2147/OTT.S227395

Figure Lengend Snippet: E6 downregulates SMG1 expression via DNMT1. ( A ) The mRNA expression of DNMT1 and SMG1 in HPV-positive cells and HPV-negative cells was detected by RT-qPCR. ( B ) The protein expression of DNMT1 and SMG1 in HPV-positive cells and HPV-negative cells were detected by Western blot. ( C ) The mRNA expression of DNMT1 and SMG1 when E6 overexpression in the FaDu and UM-SCC-4 cells was detected by RT-qPCR. ( D ) The mRNA expression of DNMT1 and SMG1 when E6 silenced in the UM-SCC-47 and UPCI-SCC-090 cells were detected by RT-qPCR. ( E ) The protein expression of DNMT1 and SMG1 when E6 overexpression in the FaDu and UM-SCC-4 cells and E6 silenced in the UM-SCC-47 and UPCI-SCC-090 cells were detected by Western blot. *P < 0.05.

Article Snippet: We used 2 HPV-negative (FaDu, UM-SCC-4) and 2 HPV-positive (UPCI-SCC-090, UM-SCC-47) HNSCC cell lines which were bought from ATCC immortalized by expression of hTERT.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Over Expression

Journal: OncoTargets and therapy

Article Title: DNMT1 Enhances the Radiosensitivity of HPV-Positive Head and Neck Squamous Cell Carcinomas via Downregulating SMG1

doi: 10.2147/OTT.S227395

Figure Lengend Snippet: Downregulation of SMG1 in HNSCC cells results in increased radiation sensitivity. ( A ) The mRNA expression of SMG1 was detected after silence SMG1 in HPV-negative HNSCC cells by RT-qPCR. ( B ) The protein expression of SMG1 was detected after silence SMG1 in HPV-negative HNSCC cells by RT-qPCR. ( C ) The sensitivity of the cells to radiation was detected by clonogenic survival assay after silence SMG1 in HPV-negative HNSCC cells. *P < 0.05.

Article Snippet: We used 2 HPV-negative (FaDu, UM-SCC-4) and 2 HPV-positive (UPCI-SCC-090, UM-SCC-47) HNSCC cell lines which were bought from ATCC immortalized by expression of hTERT.

Techniques: Expressing, Quantitative RT-PCR, Clonogenic Cell Survival Assay

Journal: Oncology Reviews

Article Title: HER3 signaling and targeted therapy in cancer

doi: 10.4081/oncol.2018.355

Figure Lengend Snippet: Anti-HER3 antibody as HER3 therapy.

Article Snippet: , , - HNSCC, colorectal cancer [ ] , Preclinical , Regeneron.

Techniques: Modification, Expressing, Mutagenesis, Amplification

Journal: Oncology Reviews

Article Title: HER3 signaling and targeted therapy in cancer

doi: 10.4081/oncol.2018.355

Figure Lengend Snippet: Summary of clinical trials, outcomes and adverse effects associated with anti-HER3 therapies.

Article Snippet: , , - HNSCC, colorectal cancer [ ] , Preclinical , Regeneron.

Techniques: Clinical Proteomics, Infection, Modification, Activity Assay, Biomarker Discovery, Expressing, Mutagenesis, Inhibition, Amplification

Journal: Microorganisms

Article Title: DNA Methylation and HPV-Associated Head and Neck Cancer

doi: 10.3390/microorganisms9040801

Figure Lengend Snippet: The molecular mechanism of induction of DNA methylation in human papillomavirus (HPV)--associated head and neck squamous cell carcinoma (HNSCC). Wild-type p53 negatively regulates DNA methyltransferase 1 (DNMT1). HPV E6 oncoprotein cause degradation of p53 and it results in the upregulation of DNMT1 ( left ). HPV E7 oncoprotein forms a complex with DNMT1 and results in DNMT1 upregulation ( middle ). MYC is upregulated in HPV-associated HNSCC and recruit DNMT3B ( right ).

Article Snippet: 528 HNSCC samples , 99 , 429 , FF , Infinium 450 k , One HPV (+) subtype out of five groups , [ ] .

Techniques: DNA Methylation Assay

Journal: Microorganisms

Article Title: DNA Methylation and HPV-Associated Head and Neck Cancer

doi: 10.3390/microorganisms9040801

Figure Lengend Snippet: DNA methylation analysis of a small sample cohort on a genome-wide scale using human papillomavirus (HPV)-associated head and neck squamous cell carcinoma.

Article Snippet: 528 HNSCC samples , 99 , 429 , FF , Infinium 450 k , One HPV (+) subtype out of five groups , [ ] .

Techniques: DNA Methylation Assay, Genome Wide

Journal: Microorganisms

Article Title: DNA Methylation and HPV-Associated Head and Neck Cancer

doi: 10.3390/microorganisms9040801

Figure Lengend Snippet: Comprehensive large-cohort DNA methylation analysis at a genome-wide scale using human papillomavirus (HPV)-associated head and neck squamous cell carcinoma.

Article Snippet: 528 HNSCC samples , 99 , 429 , FF , Infinium 450 k , One HPV (+) subtype out of five groups , [ ] .

Techniques: DNA Methylation Assay, Genome Wide, Mutagenesis